Bacterial densities and PCR-Southern blot hybridization detection of plasmid replicon-specific sequences belonging to the five IncP-1 subgroups, IncP-7 and IncP-9.

Hybridization signal: (+++) very strong, with exposure time up to five minutes; (++) strong, with exposure time up to one hour; (+) weak, with exposure time up to three hours; (−) none, with exposure time of more than three hours; (/) not analyzed.