Comprehensive Analysis of Proteins Secreted by Trichophyton rubrum and Trichophyton violaceum under in Vitro Conditions

Dermatophytes cause most superficial mycoses in humans and animals. Their pathogenicity is probably linked with the secretion of proteins degrading keratinised structures. Using 2D-PAGE and a shotgun mass spectrometry approach, we identified 80 proteins from Trichophyton rubrum and Trichophyton violaceum secretomes, under conditions mimicking those in the host. Identified proteins included endo- and exoproteases, other hydrolases, and oxidoreductases. Our findings can contribute to a better understanding of the virulence mechanisms of the two species and the different types of infection they cause. Keywords: dermatophyte • secreted proteins • secretome • two-dimensional electrophoresis • tandem mass spectrometry • expressed sequence tags