mRNA expression data from whole trachea dissected from either wild-type mice or Scnn1b-Tg mice at post-natal days 0, 3, 10, and 42

Scnn1b-Tg mice overexpress the beta subunit of the epithelial sodium channel (Scnn1b) in airway Club cells. The general phenotype of these mice is described in three published manuscripts (Mall et al. 2004, Nature Medicine, 10(5):487-93; Mall et al. 2008, Am J Respir Crit Care Med. 177(7):730-42; Livraghi-Butrico et al. 2012, Physiol. Genomics 44(8):470-84; and Livraghi-Butrico et al. 2012, Mucosal Immunology 5(4):397-408). Briefly, overexpression of the Scnn1b transgene in airway Club cells leads to hyperabsorption of sodium from the airway surface liquid, which causes airway surface liquid and mucus dehydration, resulting in reduced mucus clearance and airway mucus obstruction. The data provided here represents mRNA expression data from dissected whole trachea (distal and proximal ends were cut 3-4 cartilage rings below the larynx and just above the bifurcation, respectively) from male WT and Scnn1b-Tg littermates (C57Bl/6N Tac background) at 4 time points [postnatal days (PND) 0, 3, 10, and 42]. Histologically, PND 0 trachea are normal, a tracheal mucus plug/obstruction develops around PND 3 and typically recedes to the intrapulmonary airways after PND 10, and the trachea is again histologically normal by PND 42. The data from the WT mice provides a global look at mRNA post-natal developmental changes, while the data from the Scnn1b-Tg line provides mRNA data that allows differential gene expression due to airway mucus obstruction to be queried. The data presented for the trachea is part of a larger body of work evaluating gene expression in whole lung, trachea, and purified macrophages. 24 Total tracheal samples were analyzed; three from each timepoint. In our manuscript, we were most interested in changes between WT and Scnn1b-Tg mice, however, the data can also be used to evaluate changes in gene expression across time (PND 0, 3, 10, and 42). It should be noted that a significant difference in RNA expression quality parameters was noted for the 10 day trachea data.Scnn1b-Tg小鼠在气道Club细胞（克拉拉细胞，Clara cell）中过表达上皮钠通道（epithelial sodium channel, Scnn1b）的β亚基。该类小鼠的通用表型已在三篇已发表文献中予以阐述：Mall等（2004，《自然·医学》（Nature Medicine），10(5):487-93）、Mall等（2008，《美国呼吸与危重症医学杂志》（Am J Respir Crit Care Med），177(7):730-42）、Livraghi-Butrico等（2012，《生理学基因组学》（Physiol. Genomics），44(8):470-84）以及Livraghi-Butrico等（2012，《黏膜免疫学》（Mucosal Immunology），5(4):397-408）。简言之，气道Club细胞中Scnn1b转基因的过表达会引发气道表面液体的钠过度吸收，进而导致气道表面液体与黏液脱水，最终造成黏液清除能力降低及气道黏液阻塞。本次提供的数据为雄性野生型（WT）与Scnn1b-Tg同窝仔鼠（背景品系为C57Bl/6N Tac）的解剖完整气管组织的mRNA表达谱，其中气管组织的取材方式为：远端于喉头下方3~4个软骨环处截断，近端于气管分叉处上方截断，采样时间点共4个：出生后第0、3、10和42天（postnatal days, PND）。组织学观察显示，出生后第0天的气管形态正常；约于出生后第3天出现气管黏液栓/阻塞，通常在出生后第10天左右消退至肺内气道；至出生后第42天时，气管组织形态恢复正常。野生型小鼠的数据集可全面呈现出生后mRNA的发育性表达变化，而Scnn1b-Tg品系的数据则可用于探究由气道黏液阻塞介导的差异基因表达情况。本次提交的气管组织数据，是针对全肺、气管与纯化巨噬细胞的基因表达整体研究的一部分。本次共分析24份气管样本，每个时间点各3份。本研究的核心关注点为野生型与Scnn1b-Tg小鼠间的基因表达差异，但该数据集亦可用于评估不同时间点（PND 0、3、10和42）的基因表达变化。需特别说明：出生后第10天的气管样本的RNA表达质量参数存在显著差异。