RNAseq anaysis of hippocamal cck cells of SMARCA3 cKO mice

Purpose: To identify gene expression changes in CCK neurons of hippocsmpus of SMARCA3 cKO mice Method: Translating Ribosome Affinity Purification (TRAP) to isolate RNA from CCK+ cells and, cDNA synthesis and next generation RNAseq using Illumina Nextseq sequencer. Results: Biostatistical analysis identified 1378 genes that were altered by SMARCA3 cKO. 4 hippocampi were pooled for one translating ribosome affinity purification sample. We used mice that were CCK cre+ crossed with L10-EGFP+ mice and SMARCA3 fl/fl and littermates without SMARCA3 fl/fl controls.本数据集旨在鉴定SMARCA3条件性敲除（cKO）小鼠海马体胆囊收缩素（CCK）神经元的基因表达变化。实验方法：采用翻译核糖体亲和纯化（TRAP）技术从CCK阳性细胞中分离RNA，随后进行互补DNA（cDNA）合成，并使用因美纳（Illumina）Nextseq测序仪开展下一代RNA测序。实验结果：经生物统计学分析，共鉴定出1378个受SMARCA3 cKO调控的差异表达基因。每个翻译核糖体亲和纯化样本需混合4个海马体组织。实验所用小鼠为CCK-Cre阳性、L10-EGFP阳性且携带SMARCA3 flox/flox纯合等位基因的个体，同时以不携带SMARCA3 flox/flox的同窝小鼠作为对照。