Biofilms, hydrophobicity, and surface, membrane-bound, and culture released LTA of Streptococcus pyogenes

abiofilm formation was determined in microtiter wells as described in Materials&Methods, biofilm formation by parental strains (wt) was the positive control and set at 100%.bhydrophobicity was determined by adhesion of streptococci to hexadecane as described in Materials&Methods, adhesion by parental strains was the control and set at 100%.cµg of LTA complexed to surface proteins was obtained by trypsin extraction of 1 ml of streptococci (OD530 = 1.0) as described in Materials&Methods.dµg of LTA obtained by phenol extraction of 1 ml of trypsinized streptococci (OD530 = 1.0) as described in Materials&Methods.eLauth, X., M. von Kockritz-Blickwede, C.W. McNamara, S. Myskowski, A.S. Zinkernagel, B. Beall, P. Ghosh, R. L. Gallo, and V. Nizet. 2008. M1 protein allows group A streptococcal survival in phagocyte extracellular traps by cathelicidin inhibition. (submitted).*indicates that the mutant is significantly different from the parent, p≤0.05 as determined with students t-test.All experiments were performed in triplicate. The mean±SD is shown.