NG Capture-C analysis of GADD45a-bound adipogenic super-enhancers

ING1b and GADD45a are nuclear proteins involved in the regulation of cell growth, apoptosis and DNA repair. We previously found that ING1b is essential to target GADD45a-mediated active DNA-demethylation via TET1 to specific loci. In order to study the physical interaction of distant GADD45a and ING1 bound regions, we performed multiplexed NG Capture-C chromatin conformation capture assay in wildtype and knockout mouse embryonic fibroblasts. Overall design: NG Capture-C analysis of multiple GADD45a-bound adipogenic super-enhancers in wildtype, Gadd45a knockout, Ing1 knockout and Gadd45a/Ing1 double-knockout (DKO) mouse embryonic fibroblasts.