Supporting data for "Chemical biology study on bacterial pseudaminic acid and human caveolin-1"

In the Pse project, biochemical enrichment and proteomic approaches are employed to identify and validate Pse‑containing glycoproteins, and to reveal host proteins that specifically interact with bacterial pseudaminic acid. The provided files contain data from the Alt4NAz, Alt4N‑photo, and Pse‑photo experiments, as well as from ELISA and MST assays. For further details, the laboratory notebooks stored in the lab should be consulted, using the dates indicated in the subfolder names as reference points.<br>In the Caveolin‑1 project, chemical protein synthesis is employed to generate homogeneous, site‑specifically phosphorylated Cav‑1 proteins that serve as molecular baits for quantitative affinity purification–mass spectrometry analysis, enabling the identification and characterization of their protein‑binding partners. The provided files contain data from LC‑MS/MS, plasmid construction, protein candidate validation, cellular transient transfection, cellular GST‑pulldown experiments, and stable cell line establishment related to the Cav‑1 project.