MeRIP-seq数据

使用DynabeadTM mRNA purification kit对300 mg的总RNA进行纯化得到poly(A)+ RNA，接着在70℃反应液中进行随机片段化。使用乙醇沉淀法纯化片段化后的RNA。吸取50 ng的片段化RNA作为Input对照组，剩余的5 μg片段化RNA进行m6A的IP富集。最终将得到的50 ng抗体富集的RNA（IP）和50 ng未富集的RNA（Input）使用KAPA Stranded mRNA Kit Illumina platform构建测序文库，使用Illumina Novaseq 测序平台进行双端测序。设置了两个重复，最终通过生物信息学分析，发现m6A显著富集于基因的终止密码子和3'UTR区域。Poly(A)+ RNA was purified from 300 mg of total RNA using the Dynabead™ mRNA Purification Kit. Subsequently, random fragmentation was carried out in a reaction mixture at 70°C, followed by purification of the fragmented RNA via ethanol precipitation. Then, 50 ng of the fragmented RNA was reserved as the Input control, while the remaining 5 μg of fragmented RNA was used for m6A immunoprecipitation (IP) enrichment. Finally, 50 ng each of the antibody-enriched RNA (IP) and non-enriched Input RNA were used to construct sequencing libraries with the KAPA Stranded mRNA Kit for the Illumina platform, and paired-end sequencing was performed on the Illumina NovaSeq platform. Two biological replicates were established, and bioinformatic analysis subsequently showed that m6A was significantly enriched in the stop codons and 3' untranslated regions (3'UTRs) of genes.

搜集汇总

背景与挑战

背景概述

该数据集为MeRIP-seq实验数据，用于研究RNA的m6A甲基化修饰，包含Input和IP组的测序数据及生物信息学分析结果。数据来源于国家重点研发计划项目，数据量为18.78 GB，质量可靠。