We established a 6mA editing system with CRISPR/dCas9, to generate the targeted 6mA writer and the targeted 6mA eraser. Whole-genome methylation sequencing was used to inverstigate the efficiency and
The conserved core domain of the TATA binding protein (TBP) interacts with multiple partners forming the complexes required for transcription by RNA Polymerases I, II and III. We use genetically modif
It has been thought that epigenetic changes underlie the evolutionary divergence of phenotype between closely related species. To study differences in DNA methylation between humans and chimanzees, we
Green is RNA FISH using a probe against XIST RNA. Red is an anti-H3K27me3 antibody. Blue is DAPI. These images were used to calculate data shown in Table 2.
