Effect of SUFU overexpression and knockdown on gene expression profile in breast cancer epithelial cells

SUFU is a negative regulator of the hedgehog signaling pathway and its abnormal expression has been found in several human cancers, whereas the role of SUFU in the malignant development of breast cancer has not been fully elucidated. We performed RNA-seq to determine the impact of SUFU overexpression and knockdown on global gene expression profile in SKBR3 and HCC1954 cells. Our results show that sufu affects ferroptosis phenotype via modulating yap-dependent ACSL4 in breast cancer cells. Overall design: SKBR3 and HCC1954 cells tranduced with lentiviruses expressing oeSUFU, shSUFU and control vector were selected for RNA extraction and transcriptomic profiling by RNA-seq.