five

WGS sequencing

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https://www.ncbi.nlm.nih.gov/sra/SRP000914
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资源简介:
The Tria Project led by the Michael Smith Laboratories at UBC and BCCA Genome Sciences Centre sequenced the ~32.5 Mb genome of Grosmannia clavigera strain kw1407 at ~50X coverage using a hybrid whole genome shotgun (WGS) sequencing method. WGS projects ACXQ00000000 and ACYC00000000 are assembled from the same sequence reads using different methodologies. For ACXQ00000000 (Sanger-454-IlluminaPA) raw Illumina PE reads were assembled using Velvet, and 454 and Sanger reads were assembled with Forge. The two sets of contigs were then combined. For ACYC00000000 (Sanger-454-IlluminaDA) there was no pre-assembly. Filtered genomic DNA reads from Sanger, 454 and Illumina were assembled using the Forge genome assembler.
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2020-08-25
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