<i>TUM-ParticleTyper 2</i>: Software and Documentation

Accurate quantification of small microplastics in environmental and food samples is a prerequisite for studying their potential hazard. Knowledge of numbers, size distributions and polymer type for particles and fibers is particularly relevant in this respect. A fully automated procedure for quantifying microplastics with Raman microspectroscopy across the entire defined size range is presented in the form of the software TUM-ParticleTyper 2. This software implements the theoretical approaches of random window sampling and on-the-fly confidence interval estimation during ongoing measurements. It also includes improvements to image processing and fiber recognition (when compared to the previous software TUM-ParticleTyper for analysis of particles/fibers &gt; 10 µm), and a new approach to adaptive de-agglomeration.