RNA in situ hybridization of SAD5-clade desaturases in Ophrys flowers.
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Additional data for Figure S1: RNA in situ hybridization of SAD5-clade desaturases (SAD5 and SAD6) in Ophrys flowers. Panel (A) shows thin sections of two individuals of O. exaltata and (B) one O. sphegodes individual; for each individual, two sections at 5 and 7 mm from the base (top) of the labellum were analysed. SAD5 antisense probe and sense control are shown for O. sphegodes. The antisense panels show black signal in the epidermal layer of O. exaltata (panel A), but not in O. sphegodes (panel B); O. exaltata antisense signal (panel A) is clearly stronger than the brownish background seen with the sense and antisense probes in O. sphegodes (panel B). This is consistent with the expression pattern reported for SAD5 [1], which is highly expressed in O. exaltata but not in O. sphegodes.
References:
1. Xu et al. (2012). PLoS Genet. 8, e1002889.
创建时间：
2016-05-20
