RNA-seq of Yra1 associated mRNAs

Gene expression results from mRNA - RNA-binding protein (RBP) interactions within messenger ribonucleoprotein (mRNP) particles. We identified heterogeniety of nuclear mRNP composition involved in mRNA biogenesis and export with different occupancy and stoichiometry of RNA-binding proteins. Especially, mRNA export adopter protein Yra1 showed large variation of its stoichiometry on single mRNPs. We identified stoichiometry of Yra1 in the nuclear cap binding complex (Cbp80) bound mRNPs and it can be dissected into two categories (one and multiple Yra1 bound mRNPs). Multiple Yra1 containing mRNPs specifically co-occupied with THO complex (Hpr1). To investigate gene dependency to form different Yra1 stoichiometry mRNPs, we performed RNA-IP experiment for Yra1 with two step purification by Cbp80/Yra1 and Hpr1/Yra1.本数据集源自信使核糖核蛋白（messenger ribonucleoprotein, mRNP）颗粒内信使RNA（mRNA）与RNA结合蛋白（RNA-binding protein, RBP）相互作用的基因表达检测结果。本研究鉴定得到参与mRNA生物发生与输出过程的核mRNP组成具有异质性，其RNA结合蛋白的占据率与化学计量比存在差异。其中，mRNA输出衔接蛋白Yra1在单个mRNP上的化学计量比差异显著。我们明确了结合于核帽结合复合物（Cbp80）的mRNP中Yra1的化学计量比，并可将其划分为两类：仅结合单个Yra1的mRNP，以及结合多个Yra1的mRNP。其中携带多个Yra1的mRNP会特异性地与THO复合物（Hpr1）共同结合。为探究不同Yra1化学计量比的mRNP形成的基因依赖性，我们以Cbp80/Yra1和Hpr1/Yra1为纯化靶点开展了两步纯化实验，并针对Yra1进行了RNA免疫沉淀（RNA-IP）实验。