Comparative transcriptome analysis reveals critical genes/pathways related yolk precursor synthesis in livers of Guangxi Ma chicken with different egg laying rate

Liver tissues of Guangxi Ma chicken from 32-week old with m performance, 50-week old with high and low laying performance, and 72-week old with high and low laying performance were collected and sequenced in quadruplicate using RNA-seq. The sequences were double-ended sequenced on the DNBSEQ sequencing platform. The sequence reads were quality controlled and then aligned with genomic sequences using HISAT2 program, quantified by featureCounts program, and gene expression levels were verified by qRT-PCR with SYBR Green detection. The results will be helpful to explore the factors that affecting laying performance from the perspective of yolk synthesis and provide a theoretical basis for improving the egg production of Guangxi Ma chicken. Liver mRNA profiles of Guangxi Ma chicken at 32-week old (C32), 50-week old with high (C50WH) and low (C50WL) laying performance, 72-week old with high (C72WH) and low (C72WL) laying performance.