Effect of a natural product derivative on gene expression signatures in 5xFAD transgenic mice

To investigate how a natural product analog mitigates AD, cortical transcriptomes from WT and 5xFAD mice were analyzed. Comparing vehicle-treated groups revealed extensive differential expression associated with neuroinflammation in 5xFAD mice. This was attenuated in the 5xFAD mice treated with the natural product, whose profiles resembled WT controls. RNA seq suggested the natural product regulated the nervous and immune systems. RNA-seq was employed to profile the effects of a natural product derivative on gene expression signatures in 5xFAD transgenic mice. Cortical tissues from five experimental groups, namely WT Veh, WT High P, 5xFAD Veh, 5xFAD Low P, and 5xFAD High P, were collected. Tissues from five mice per group underwent RNA extraction and subsequent transcriptome sequencing (RNA-Seq).