Early life exposure to N-nitrosamine drives genotoxicity, mutagenesis, and tumorigenesis in DNA repair-deficient mice

Supporting data for: Volk et al. (2026) "Early life exposure to N-nitrosamine drives genotoxicity, mutagenesis, and tumorigenesis in DNA repair-deficient mice," Nature Communications. N-Nitrosodimethylamine (NDMA) is a probable human carcinogen found in contaminated pharmaceuticals and drinking water. Using DNA repair-deficient (Aag-/-;Mgmt-/-) and wild-type mice, we systematically compared the effects of NDMA exposure in juveniles and adults. This dataset includes raw mass spectrometry data (DNA adduct quantification by LC/ESI-MS/MS), NDMA dose quantification and drinking water consumption data, GPT delta assay results, western blot scans and quantification, immunofluorescence analysis results, RaDR foci and clonal expansion analysis results, and source data for all figures. Full experimental metadata, sample lineage, and study documentation are available at FairdomHub (https://fairdomhub.org/studies/1367). Sequencing data (FASTQ) are available at NCBI SRA (BioProject PRJNA1422080). Duplex sequencing variant calls and RNAseq analysis code/data are available on Code Ocean (DOI: 10.24433/CO.3406581.v1). Please see the included README.txt for a description of each file in this deposit.