Murine CD4+ T cells: naive vs. experimental autoimmune uveitis

miRNA expression profiling of CD4+ T cells comparing naïve mice and experimental autoimmune uveitis (EAU) mice. EAU was induced by immunization of retinal antigen (IRBP1-20) in complete Freund’s adjuvant (CFA). CD4+ T cells were isolated and purified from the spleen and draining lymph nodes 13 days after immunization. Naïve CD4+ T cells vs. EAU CD4+ T cells. 6 control, 6 EAU, independently harvested. RNAs from CD4+ T cells of six mice were pooled as one sample. One sample was generated from naïve CD4+ T cells and one sample was from EAU CD4+ T cells. Totally, 2 samples were collected and analyzed.