Dynamic and distinct histone modifications facilitate human trophoblast lineage specification

The placenta serves as an essential organ for nurturing fetal growth throughout pregnancy. Histone modification is a crucial regulatory mechanism involved in numerous biological processes and development. Nevertheless, there remains a significant gap in our understanding regarding the precise mechanisms and extent to which various histone modifications influence the critical process of trophoblast lineage differentiation, which is fundamental to placental development. Here, we conducted a comprehensive mapping of H3K4me3, H3K27me3, H3K9me3, and H3K27ac loci during trophoblast stem cells (TSC) differentiation into syncytiotrophoblasts (ST) and extravillous trophoblasts (EVT) to investigate the regulatory roles and dynamic changes in histone modifications in trophoblast lineage specification. Overall design: ChIP-seq were performed for various histone modfication (H3K4me3, H3K27me3, and H3K9me3, and H3K27ac) in human trophoblast stem (TS) cells, cyncytiotrophoblat, Extravillous trophoblast. Input was sequenced for a control.