Drug-induced transcriptome variation in PSC-derived cardiomyocytes

This dataset provides a curated summary of drug-induced transcriptomic variation in serum-free human pluripotent stem cell-derived cardiomyocytes (WA09/H9). The dataset aggregates differential expression outputs across multiple pharmacological perturbations relevant to cardiometabolic aging and geroprotection, including mTOR inhibition, AMPK modulation, senolytic interventions, and hormonal signaling regulators. The experimental model consists of serum-free PSC-derived cardiomyocytes with a senescent reference state. Differential expression was computed using DESeq2, and the dataset compiles gene-level log2 fold changes and adjusted p-values across all compound–dose contrasts relative to senescent cardiomyocyte controls. The primary data product is a long-format gene-level table containing differential expression metrics across all perturbations. A companion summary table provides compound-level DEG counts and reversal metrics to facilitate comparative pharmacotranscriptomic analyses. This dataset is suitable for:- pharmacotranscriptomic benchmarking- senescence reversal studies- geroprotector screening- integration with epigenetic or multi-omics datasets Analysis was performed using standardized RNA-seq pipelines with DESeq2-based differential expression. Significance thresholds applied in summaries were adjusted p-value < 0.05 and |log2 fold change| ≥ 1. This release represents version 1.0 of the dataset. This dataset is associated with a previously published Data Management Plan (DMP) describing data governance, storage, and sharing strategies for the project: Megnis, K. (2025). Therapy-induced transcriptome variations to promote heart cell senescence reversal (Version 1). Zenodo. https://doi.org/10.5281/zenodo.16309877 The DMP defines the FAIR data framework under which this dataset was generated and released. This research was supported within the framework of the European Union’s Recovery and Resilience Mechanism project No.5.2.1.1.i.0/2/24/I/CFLA/001 "Consolidation of the Latvian Institute of Organic Synthesis and the Latvian Biomedical Research and Study Centre" (ANM_K_PG_17).