Comparative analysis of cellular heterogeneity in the LAM lung using scRNA-seq. Comparative analysis of cellular heterogeneity in the LAM lung using scRNA-seq

Investigate detailed cellular composition of the LAM lung compared to the age and sex matched healthy control, in order to isolate LAM-lung-specific cellular states or subtypes. Overall design: Age-and sex-matched donor lung explants from control healthy and LAM patients were subjected to the single cell transcriptomocs analysis in parallel (n=1 each). Sample prepartion included creating a single cell suspension and immune cell pre-depletion using human anti-CD45 MACS beads. 10% of CD45+ cells were addded back to the samples to comprise 20K cells for the 10x genomics capture. Obtained libraries were sequenced in 2 repeats using Illumina HiSeq