Specific COX-2 inhibitor confers complete protection against both PAF-induced lethality and delayed death due to PAF and hyperactivated TLR-4.

Mice were divided into 7 groups containing 6 animals each. Three groups received an intraperitoneal injection of NS-398 (20 mg/kg body wt) 30 min before receiving PAF, LPS, or a combination of both. NS-398 (20 mg/kg body wt) completely abolished PAF-induced sudden death and enhanced the cross-tolerance effect exerted by the high dose of LPS (20 mg/kg). The animals were monitored for survival for up to 6 days. The results are representative of 3 individual trials.