Additional file 1: of Evaluation of commercially available small RNASeq library preparation kits using low input RNA

Table S1. Percentage of input reads aligned to the human transcriptome, human rRNA, UniVec contaminant sequences and discarded because they are too short (< 15 nts) and unmapped to the human transcriptome. Table S2. Median (Inter-quartile range) of percentage of input reads aligned to the human transcriptome, human rRNA, UniVec contaminant sequences. Table S3. Percentage of reads aligned to the human transcriptome to each RNA biotype for all samples. Table S4. Median (Inter-quartile range) of percentage of input reads aligned to different RNA biotypes between the three sequencing kits. Table S5. Median (IQR) of percentage of input reads aligned and comparison of input amount of RNA. Table S6. Median (IQR) of percentage of input reads aligned and comparison between the two sites for the two input amounts of RNA. Table S7. Median (IQR) of number of miRNAs greater than 10 counts detected in at least 25% of the samples between the two sites for the two input amounts of RNA. Table S8. Pearson’s and Spearman’s correlation coefficient by tissue, kit and input amount. Table S9. Kit specific miRNAs found in each tissue for each kit. The top 5 miRNAs for each tissue that have expression greater than 10 RPM in one kit, but less than 5 RPM in the other two are presented for each tissue and kit. Table S10. miRNAs included on the custom made FirePlex Panel. The columns denote the number of samples that had above detection-limit expression in each tissue. Table S11. Database of RNA biotypes used. (XLSX 89 kb)