Hypotaurine reduces glucose-mediated vascular calcification

To study the underlying molecular mechanisms driving vascular calcification, we analyzed the transcriptome of calcium-phosphate calcified human coronary aortic smooth muscle cells cultured with zero, low, and high glucose for 3 days. Human primary coronary artery smooth muscle cells from three independent donors were cultured in a control medium (5.5 mM glucose) or calcified in a calcium-phosphate medium (CaP) with 0 mM, 5.5 mM and 25 mM glucose.