Untargeted LC–MS/MS metabolomics of femoral/tibial bone tissues from OVX-induced osteoporosis mice treated with DCNPs, ALN, Mix, PBS treatment or healthy controls

This dataset contains untargeted metabolomics profiles acquired by UPLC–HRMS (positive and negative ESI modes) from femur/tibia bone tissues of OVX-induced osteoporosis mice across five groups.Healthy (sham) (Labelled <i>NC</i> in data file)OP + PBS (PBS)(Labelled <i>OVX </i>in data file)OP + Alendronate (ALN)(Labelled <i>ALN </i>in data file)OP + Mix (EA + ALN + PEI + CaCl2 without 2-FPBA)(Labelled <i>Mix </i>in data file)OP + DCNPs (EA + ALN + PEI + 2-FPBA + CaCl2) (Labelled <i>NPs </i>in data file)<br>Female C57BL/6J mice were used. The goal is to characterize DCNP-mediated transcriptional reprogramming in bone, including activation of JAK2–STAT3 signaling and suppression of inflammatory lipid metabolism pathways identified in the companion manuscript<br>Provider<br>Untargeted metabolomics was performed by Lianchuan Company (commercial service provider).Assay and platformAssay type: Untargeted UPLC–HRMS/MS (ESI+ and ESI−)UPLC: Waters ACQUITY UPLC with ACQUITY UPLC T3 column (100 × 2.1 mm, 1.8 μm), 40 °CMobile phases: A = water + 5 mM ammonium acetate + 5 mM acetic acid; B = acetonitrileGradient: 0–0.8 min 2% B; 0.8–2.8 min 2–70% B; 2.8–5.6 min 70–90% B; 5.6–6.4 min 90–100% B; 6.4–8.0 min 100% B; 8.0–8.1 min 100–2% B; 8.1–10.0 min re-equilibration at 2% BFlow rate: 0.3 mL/minMS: SCIEX TripleTOF 6600; IDA mode; mass range 60–1200 m/z; ESI+ 5000 V / ESI− −4500 V; source temp 500 °CQC: pooled QC injected every 10 samples; calibration every 20 samplesSamplesSpecies/strain/sex: Mus musculus, C57BL/6J, femaleTissue: Femur and tibia (bone)Groups and size: 5 groups × n = 6 biological replicates per group = 30 samples total (+ pooled QC)Model and treatments: variectomy (OVX) osteoporosis model; sham for HealthyEthics: Approved by the Laboratory Animal Ethics Committee of the First Affiliated Hospital of Wenzhou Medical University (No. WYYY-AEC-2025-008)