RNA-seq of livers from glucagon receptor knock-out (GcgrKO) mice compared to wildtype littermates

Glucagon is a key regulator of glucose homeostasis, amino acid catabolism, and lipid metabolism. Glucagon receptor knock-out (GcgrKO) mice have slightly reduced blood glucose levels whereas plasma levels of amino acids are vastly increased reflecting disruption of hepatic amino acid catabolism. To dissect the molecular mechanisms underlying this effect, RNA sequencing of livers from male GcgrKO mice and wild-type littermates were performed. The mice were 10 weeks of age and were subjected to a short-term fast of 4 h before anesthesia with 2.5% isoflurane.胰高血糖素（Glucagon）是葡萄糖稳态、氨基酸分解代谢以及脂质代谢的关键调控因子。胰高血糖素受体敲除（GcgrKO）小鼠的血糖水平轻度降低，但其血浆氨基酸水平显著升高，这反映出肝脏氨基酸分解代谢受到破坏。为解析该效应背后的分子机制，本研究对雄性GcgrKO小鼠及其野生型同窝仔鼠的肝脏组织开展了RNA测序（RNA sequencing）。实验小鼠均为10周龄，在使用2.5%异氟烷进行麻醉前，均接受了4小时的短期禁食处理。