Sputum_analysis_LSRII_cytometer
收藏NIAID Data Ecosystem2026-03-12 收录
下载链接：
http://flowrepository.org/id/FR-FCM-Z3MJ
下载链接
链接失效反馈官方服务：
资源简介：
To determine whether our method for sputum processing can be applied to different flow cytometric platforms.
Conclusion:
Using our methods, we were able to successfully dissociate sputum into a single cell suspension into live, viable cells that were stained with blood and epithelial lineage markers and analyzed by three flow cytometric platforms.
Notes:
Three-day sputum collection from persons aged 55-74 with at least a 30 pack-year smoking history at high risk of developing lung cancer. This experiment was used as a proof of concept of our protocol for a methods manuscript. No treatments or experimental conditions were applied to the samples. LSR II cytometer was checked prior to each analysis session with BD FACSDiva™ CS&T Research beads. The LSR II PMTs are adjusted and checked prior to each use using SPHERO™ Rainbow Calibration Particles. The presence of alveolar macrophages was verified to determine the sample derives from the lung.
创建时间：
2021-06-01
