raw data for Genotyping

Gel image used in Figure 1<br>Genomic DNA from human iPSCs amplified for the APOE locus, then digested with HhaI enzyme. Run on 3% agarose gel.<br>Genotype of the cell line used in this study was compared to that of another cell line maintained in the lab that was known to be homozygous for APOE3.<br>Genotyping method based on Henderson et al. (2002).Henderson, J.N., Crook, R., Crook, J., et al. 2002. Apolipoprotein E4 and tau allele frequencies among Choctaw Indians. <i>Neuroscience Letters</i> 324(1), pp. 77–79.

图1所用的凝胶电泳图像
从人类诱导多能干细胞（human induced pluripotent stem cells, iPSCs）中提取基因组DNA，针对载脂蛋白E（apolipoprotein E，APOE）基因座进行扩增，随后经HhaI限制性内切酶酶切，于3%琼脂糖凝胶中进行电泳分离
将本研究所用细胞系的基因型，与本实验室保藏的另一株已知为APOE3纯合子的细胞系基因型进行比对
本研究的基因分型方法参考自Henderson等人（2002）的研究：Henderson J.N.、Crook R.、Crook J.等，2002年。《乔克托印第安人群中载脂蛋白E4与tau等位基因频率》，《神经科学快报（Neuroscience Letters）》第324卷第1期，第77-79页