RNA-seq expression profiling of terminal end buds and ducts from mouse mammary glands

The mammary glands of adult female mice were divided into ductal tissue and terminal end buds (TEBs). Basal and luminal epithelial cells were FACS sorted and RNA extracted for 75bp paired-end RNA-seq profiling using an Illumina NextSeq 500 sequencer. Tissue was extracted from three independent mouse pools resulting in n=3 biological replicates for basal cells from ducts, basal cells from TEBs, and luminal cells from TEBs and n=2 biological replicates for luminal cells from ducts.成年雌性小鼠的乳腺被划分为导管组织与末端芽体（terminal end buds, TEBs）。采用荧光激活细胞分选（Fluorescence Activated Cell Sorting, FACS）分离基底上皮细胞与腔上皮细胞，提取RNA后使用Illumina NextSeq 500测序仪开展75bp双端RNA测序（RNA-seq）转录组分析。实验所用组织取自3组独立的小鼠混合样本，其中导管来源基底细胞、TEB来源基底细胞以及TEB来源腔上皮细胞均设置3次生物学重复（n=3），导管来源腔上皮细胞设置2次生物学重复（n=2）。