16S-rRNA sequencing and analysis due to different NGS. bioreactor metagenome

The analysis of environmental microbial communitues currently relies on a PCR-dependent amplification of genes, the 16S-rRNA entailing species identify features.This approach has enabled to build a vast portion of our knowledge in microbiology throughout different environments but it is susceptible of biases that depend on the level of primer matching to their target regions and does not convey information on the actual level of physiological activity of each taxon. An alternative approach represented by the direct sequencing of 16S-ribosomal RNA without any primer was compared to those obtained by a conventional PCR-based amplicon pyrosequencing. Different stages during the development of an ANAMMOX-based bioreactor were considered as reference-systems.