miRCURY LNATM microRNA Array (7th Gen) profiling of monocyte-derived osteoclasts and macrophages.. Homo sapiens

To examine the miRNA changes that occur during osteoclast differentiation we used the miRCURY LNATM microRNA Array (7th Gen) to profile monocyte-derived osteoclasts and monocyte-derived macrophages. Of the 2046 probes present on the assay, we detected expression of ~400 (20%) miRNAs across all samples. Overall design: CD14+ monocytes were isolated from the peripheral blood of 4 normal, healthy volunteers and differentiated into osteoclasts or macrophages in vitro. Total RNA was isolated from these cells at 0, 24, 72, 144, and 288 hours of culture. RNA was profiled using the Qiagen miRCURY LNATM microRNA Hi-Power Labelling Kit and miRCURY LNATM microRNA Array (7th Gen).