Genome-wide profiling of transcription factor activity in primary liver cancer using single cell ATAC sequencing

Genome wide chromatin profiling of 16 primary liver cancer patients was performed using scATAC-seq. The aim of this study was to assess transcription factor activity in malignant and microenviornment component cells of human primary liver cancers. scATAC-seq profiles reveal similar microenvironment composition, yet malignant cells have high levels of heterogeneity in TF activation within and between patients. Further analysis of transcription factor activity determined nuclear receptors as crucial for maintaining hepatocytic features in HCC, while ETS factors are crucial for maintaining cholangiocytic features in iCCA. Genome wide chromatin profiling of 16 primary liver cancer patients was performed using scATAC-seq.