A gene regulatory network of the endothelial-to-hematopoietic transition [ATAC-seq]

In vertebrate embryos, hematopoietic stem cells (HSCs) are generated from a subset of the aortic endothelium, the hemogenic endothelium (HE), via a process called endothelial-to-hematopoietic transition (EHT). The transcriptional mechanisms by which EHT is controlled, and how HE initiates hematopoietic differentiation, are not fully understood. In this study, we generated mini-bulk RNA-seq and ATAC-seq datasets from EHT populations in the E8.5 to E10.5 mouse embryonic dorsal aorta, and integrated these data to construct a predictive gene regulatory network underlying EHT. Overall design: ATAC-seq data generated from pools of 1500 cells. Data generated from EHT cell populations from E8.5, E9.5, and E10.5 mouse dorsal aorta. Biological replicates are included. EC - endothelial cell; HE - Hemogenic endothelium, HPC - hematopoietic progenitor cell.