CTAP ChIP Seq

This project mainly focuses on revealing new components and interactions within a transcriptional network underlying establishment and maintenance of the pluripotent state in mouse embryonic stem cells. We will map the genetic regulatory nodes and networks that control the activity of embryonic stem cells and the process of differentiation to specific cell fate by applying Chromatin Immunoprecipitation followed by Sequencing (ChIP-Seq) and analyze interested protein complex’s DNA binding patterns. We have established a high throughput mythology for in vivo protein tagging to generate mouse ES cell lines with specific knocked-in affinity tags. At present we are in the process of evaluating the tags in protein affinity purification and also in chromatin immunoprecipitation experiments. To identify the DNA sequences from the chromatin immunoprecipitation, with higher resolution, fewer artifacts, greater coverage and a larger dynamic range, the on-site Illumina/Solexa sequencing technology will be applied to sequence the pulled-down DNA samples, with the service fully supported with library preparation, pair-end sequencing, followed by genomic DNA mapping by Bioinformatics group after the pulled-down DNA sequence reads obtained.