BIOLOGICAL ACTIVITY OF A THERAPEUTIC PASTE PREPARED FROM MEDICINAL AND AROMATIC PLANTS AS A BIOACTIVE FOOD SUPPLEMENT

This article describes the bioactive therapeutic ingredients used in the formulation of the dietary supplement called “ASTOL” formulated from nettle (Urtica dioica) and sea buckthorn (Hippophae rhamnoides). This research focuses on the composition of the supplement chemically, and underlines the presence of over 60 essential macro- and microelements that are needed for balancing metabolism and maintaining physiological functions. One specific element to be evaluated in this research was the antioxidant activity of “ASTOL” that included the inhibiting of adrenaline autooxidation. Based on the testing results, the supplement has high antioxidant activity as shown in above 10% results, indicating possible active properties towards reducing oxidative stress and free radicals. Furthermore, the article explains toxicological and pharmacological findings from the evaluation as part of the work of “ASTOL” safety analysis. Acute toxicity assays were carried out, using the OECD 2002 process and administered orally in designated dosages. This study found that the LD50 value in the supplement is over 2000 mg/kg, proving the lower degree of toxicity for this material, as well as a very high tolerance to toxicity. Additional evaluations of its anti-inflammatory activity also produced encouraging results, suggesting “ASTOL” represents a valuable therapeutic candidate for inflammatory disorders.