Expression data from kidneys of rats with and without glomerulonephritis

To obtain insight into molecular processes during GN pathogenesis, mRNA expression was investigated in WKY and SD kidneys. The immunopathological processes during GN are still not fully understood and likely involve both innate and adaptive immunity. In the present study, several hundred mRNAs were found deregulated, which functionally were mostly associated with inflammation and regeneration. The ß-chain of the major histocompatibility complex class II RT1.B (Rt1-Bb) and complement component 6 (C6) were identified as two mRNAs differentially expressed between WKY and SD rat strains which could be related to known different susceptibilities to NTS of different rat strains; both were increased in WKY and decreased in SD rats. Increased Rt1-Bb expression in WKY rats could indicate a stronger and more persistent cellular reaction of the adaptive immune system in this strain, in line with findings indicating adaptive immune reactions during GN. The complement cascade is also known to be essential for GN development, especially terminal cascade products like C6.为深入探究肾小球肾炎（Glomerulonephritis, GN）发病过程中的分子机制，本研究对WKY与SD大鼠肾脏内的信使RNA（mRNA）表达水平进行了检测分析。目前学界对肾小球肾炎的免疫病理过程尚未完全阐明，该过程可能同时涉及固有免疫与适应性免疫应答。本研究中，共发现数百个信使RNA分子存在表达失调，其功能主要与炎症反应及组织再生相关。 本研究鉴定出主要组织相容性复合体II类RT1.B的β链（Rt1-Bb）与补体成分6（C6）这两种信使RNA，二者在WKY与SD大鼠品系间存在差异表达；该结果或与不同大鼠品系对肾毒性血清（Nephrotoxic Serum, NTS）的已知易感性差异相关：二者在WKY大鼠体内表达上调，而在SD大鼠体内表达下调。WKY大鼠体内Rt1-Bb表达上调，提示该品系的适应性免疫系统可产生更强且更持久的细胞免疫应答，这与此前关于肾小球肾炎过程中存在适应性免疫应答的研究结果一致。补体级联反应同样被证实对肾小球肾炎的发生发展至关重要，尤其是级联反应末端产物如C6。