METHOD FOR OBTAINING GLYPHOSATE-RESISTANT RICE BY SITE-DIRECTED NUCLEOTIDE SUBSTITUTION

Plants by replacing threonine (T) with isoleucine (I) at position 8 of the amino acid sequence of the conserved region of the endogenous EPSPS protein of the target plant， and proline (P) with serine (S) at position 12， A method for obtaining glyphosate-tolerant rice by site-specific nucleotide substitution is provided， comprising the step of obtaining a glyphosate-tolerant plant. A method for achieving site-specific nucleotide substitution and fragment substitution that replaces regions between gRNA sites by designing two gRNA sites using the CRISPR-mediated NHEJ pathway is provided. A method for obtaining glyphosate-resistant rice by a site-directed nucleotide substitution is provided， which comprises the following steps: only substituting threonine (T) at position 8 of the amino acid sequence of a conserved region of endogenous EPSPS protein of a target plant with isoleucine (I)， and substituting proline (P) at position 12 with serine (S) to obtain a plant， i.e.， a glyphosate-resistant plant. A method capable of generating a site-directed nucleotide substitution and a fragment substitution utilizing a CRISPR-mediated NHEJ pathway to substitute a region between two gRNA sites by designing the two gRNA sites is provided. A method for obtaining glyphosate-resistant rice by a site-directed nucleotide substitution is provided， which comprises the following steps: only substituting threonine (T) at position 8 of the amino acid sequence of a conserved region of endogenous EPSPS protein of a target plant with isoleucine (I)， and substituting proline (P) at position 12 with serine (S) to obtain a plant， i.e.， a glyphosate-resistant plant. A method capable of generating a site-directed nucleotide substitution and a fragment substitution utilizing a CRISPR-mediated NHEJ pathway to substitute a region between two gRNA sites by designing the two gRNA sites is provided.