Inhibition of lysine demethylase 6B blocks the development of ASXL1 truncation-mediated myeloid malignancies [RNA-seq]

To investigate the role of KDM6B in the pathogenesis of truncated ASXL1-mediated myeloid malignancies, we purified the Lin-cKit+ cells from bone marrow of WT, Kdm6b?/+, Asxl1Y588XTg, and Asxl1Y588XTg;Kdm6b?/+ mice and performed the bulk RNA-seq to study the dysregulated genes due to the reduction of Kdm6b in Asxl1 mutant context. Overall design: RNA-seq for bone marrow Lin-cKit+ cells from WT (n=3), Asxl1Y588XTg (n=3), Kdm6b?/+ (n=3) and Asxl1Y588XTg;Kdm6b?/+ (n=3) mice.