Quantitative Proteomics of mPFC Tissue in a Vascular Cognitive Impairment Mouse Model
收藏国家人口健康科学数据中心2026-06-06 收录
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资源简介：
Deep quantitative proteomic profiling of bilateral medial prefrontal cortex (mPFC) from control (ctrl), vascular cognitive impairment (VCI, model) and EVs treatment (test) mice (3 biological replicates each). Proteins extracted with RIPA + inhibitors, quantified by BCA, processed via FASP, digested with trypsin, labeled with TMT-16plex, fractionated by high-pH RP, and analyzed on nanoLC-Q Exactive HF-X (DDA, 120k/15k resolution). Raw files searched against Mus musculus UniProt in PD 3.0/Mascot (FDR ≤1%). Differentiation criteria: fold-change >1.2, p<0.05 (Perseus).
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北京蛋白质组研究中心创建时间：
2026-02-10
