Single cell analysis of CD45-positive immune cells infiltrating the central nervous system of mice undergoing experimental autoimmune encephalomyelitis.
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https://www.ncbi.nlm.nih.gov/sra/ERP134487
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C56BL/6 mice (10 to 12-week-old) were immunized subcutaneously at the base of the tail with 100 µg of MOG (35â55) emulsified in complete Freund's adjuvant containing 500 µg of Mycobacterium tuberculosis. Pertussis toxin (200 ng; List Biological Laboratories) was injected intravenously at days 0 and 2 post-immunization. At day 17 post immunization, central nervous system CD45-positive immune cells were isolated and loaded onto a single channel of a 10x Chromium chip for each sample.
创建时间:
2023-10-13



