Investigation of the physiological functions of PRDM6

PRDM6 belongs to the PRDM family of transcriptional repressors that all contain a PR domain and Zinc finger domains. Prdm6 has transcriptional repressor activity. Prdm6 may regulate factors that are involved in the differentiation / proliferation of smooth muscle cells, promoting proliferation. PRDM6 knockouts are embryonic lethal around 12.0 dpc knockout embryos eventually develop edema: cardiovascular defect vascularization defect in the yolk sac: dysorganized angiogenesis Keywords: cDNA array, yolk sac, murine embryo E10.5 6 homozygote samples (yolk sacs), age 10.5 days, hybridized against a pool of of 6 wildtype samples in 12 replicates including 6 dye swap experiments.PRDM6属于PRDM家族转录抑制因子（transcriptional repressors），该家族所有成员均含有PR结构域（PR domain）与锌指结构域（Zinc finger domains）。Prdm6具备转录抑制活性，其可能调控参与平滑肌细胞分化/增殖的相关因子，并促进细胞增殖。PRDM6基因敲除（knockouts）小鼠胚胎在约12.0 dpc时致死，敲除胚胎最终会出现水肿、心血管缺陷、卵黄囊血管化缺陷及血管生成紊乱。关键词：cDNA芯片（cDNA array）、卵黄囊（yolk sac）、鼠胚E10.5；实验样本为6份发育时长10.5天的纯合子卵黄囊样本，以6份野生型样本混合池为对照，共设置12次重复实验，其中包含6次染料交换（dye swap）实验。