Mitochondrial complex I activity in microglia sustains neuroinflammation - CyTOF data and analysis

This repository documents the raw data and analysis of the CyTOF results reported in: Mitochondrial reverse electron transport in myeloid cells perpetuates neuroinflammation L. Peruzzotti-Jametti, C.M. Willis, R. Hamel, G. Krzak, J.A. Reisz, H.A. Prag, V. Wu, Y. Xiang, A.M.R. van den Bosch, A.M. Nicaise, L. Roth, G.R. Bates, H. Huang, A.E. Vincent, C. Frezza, C. Viscomi, J.C. Marioni, A. D’Alessandro, Z. Takats, M.P. Murphy, S. Pluchino bioRxiv 2024.01.03.574059; doi: https://doi.org/10.1101/2024.01.03.574059 Contents: Ndufs4.zip /Rcode/ --- notebooks used for the analysis and figure generation /data/ndufs4_lymphoid/ --- fcs files for lymphoid gating /data/ndufs4_myeloid/ --- fcs files for myeloid gating /data/Ndufs4_metadata.xlsx --- metadata /data/suspension_mass_cytometry_panel.xlsx --- CyTOF panel small_molecule.zip /Rcode/ --- notebooks used for the analysis and figure generation /data/cleaned_and_gated/lymphoid/ --- fcs files for lymphoid gating (after Gd correction) /data/cleaned_and_gated/myeloid/ --- fcs files for myeloid gating (after Gd correction) /data/cleaned_and_gated/small_molecules_metadata_lymphoid.xlsx --- metadata /data/cleaned_and_gated/small_molecules_metadata_myeloid.xlsx --- metadata /data/cleaned_and_gated/suspension_mass_cytometry_panel.xlsx --- CyTOF panel /data/raw/ --- fcs files (before Gd correction)