RNA-seq of mouse primary cortical neurons transfected with a synthetic mimic of 5'tiRNA (Gly-GCC) or a scrambled control

tRNA-derived stress-induced RNAs (tiRNAs) are a new class of small non-coding RNA that have emerged as important regulators of cellular stress responses. tiRNAs are derived from specific tRNA cleavage by the stress-induced ribonuclease angiogenin (ANG). Loss-of-function mutations in the ANG gene are linked to amyotrophic lateral sclerosis (ALS), and elevated levels of specific tiRNAs were recently identified in ALS patient serum samples. However, the biological role of tiRNA production in neuronal stress responses and neurodegeneration remains largely unknown. This study aimed to evaluate the genome-wide regulation of neuronal stress responses by an ALS-associated tiRNA; 5'tiRNAGly-GCC. To this end, we performed whole-transcript RNA-seq on mouse primary cortical neurons transfected with either a synthetic mimic of endogenous 5'tiRNAGly-GCC or a scrambled tiRNA control. This dataset contains the RNA-seq data used to identify the effects of 5'tiRNAGly-GCC on global gene expression in primary neurons.