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Single-molecule and bulk fluorescence and biolayer interferometry on the NAD-II riboswitch and its interactions with NMN

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NIAID Data Ecosystem2026-05-02 收录
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http://datadryad.org/dataset/doi%253A10.5061%252Fdryad.4b8gthtnw
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The Class II NAD+ riboswitch is a bacterial RNA that binds ligands containing nicotinamide. Herein, we report a fluorescence and biolayer interferometry study of riboswitch interactions with β-NMN. The results reveal a shift in the prevalence of a pseudoknot structure in the presence of ligand and Mg2+. Methods Bulk fluorometry, single-molecule microscopy, gel electrophoresis, biolayer interferometry data on the NAD-II riboswitch.
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2024-12-05
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