NCBI Organism:Mus musculus, ; Cell Types:, , ;

<b>MiniSOG+ ipRGC axon labeling in the olivary pretectal nucleus (OPN)</b><br>We have labeled ipRGCs with a genetically encoded and membrane-tethered version of miniSOG and systematically mapped their axonal arbors within 3D SBEM volumes of mouse ON, SCN, OPN, vLGN, IGL, and dLGN. Although ipRGCs have been classified into up to 6 different subtypes based on their dendritic arbors in the retina, there is no conclusive evidence that any subtype specifically and exclusively targets any brain region. By quantitatively determining the ultrastructural features of ipRGC synaptic contacts and intracellular machinery within these volumes, we discovered salient target-specific synaptic properties of ipRGCs.

<b>MiniSOG+ ipRGC轴突在橄榄核顶盖核（OPN）中的标记</b><br>本研究利用基因编码并膜连接的MiniSOG技术对ipRGC进行标记，并在小鼠的视神经、下丘脑、橄榄核顶盖核、外侧膝状体、内侧膝状体和深层膝状体等3D亚微米电子显微镜（SBEM）体积中系统地绘制了其轴突树突。尽管根据视网膜中的树突树突，ipRGC已被划分为多达6个不同的亚型，但尚未有确凿的证据表明任何亚型专门且唯一地靶向任何脑区。通过定量分析这些体积中ipRGC突触接触和细胞内机制的亚显微结构特征，我们发现了ipRGC突触的显著靶特异性特性。