KM1709_Mesoscope_B12_Incubations_HILICMode

KM1709 Mesoscope B12 Incubations HILIC (Positive and Negative). These are targeted metabolite peak areas, representative of abundance values taken.The samples were taken from each of the two dipoles of a mesoscale eddy near Station Aloha and incubated for four days. Incubation #1 was the cyclonic dipole, while Incubation #2 was the anticyclonic dipole. Six conditions were created for each of the samples. T0: In situ condition at the start of the experiment, aka time 0. Water that was filtered, not incubated, and frozen. Control (Tfinal): 100% in situ water from 25 m, incubated and then filtered. Deep Sea Water mix: 10% Deep Sea Water (DSW) taken from 700 m, filtered and evenly mixed with 90% in situ water from 25 m. This mixture was then incubated and filtered. +B12: 25 m water spiked with 800 ul of B12 for a final concentration of ~240 pM, incubated, filtered. - B12: 25 m water spiked with trace metals (iron, Si, PO4, NO3) and no B12, incubated, filtered. +DMB, -B12: 25 m water with the same conditions of the -B12 state, but with an added spike of 5,6-dimethylbenzimidazole (DMB). +DMB: 25 m water spiked with 800 ul of DMB for a final concentration of 240 pm, incubated, filtered. Samples from KM1709 (MESO-SCOPE 2017) were collected and produced by the Ingalls Lab as part of the Simons Gradients project with support from the Simons Foundation.