Untargeted LC-HRMS-based metabolomic profiling reveals distinct metabolomic profiles in patients with metabolic dysfunction-associated steatotic liver disease (MASLD)

We analyzed liver tissues, liver secretome (conditioned medium), and blood plasma samples from 109 obese patients undergoing bariatric surgery spanning early stages of metabolic dysfunction-associated steatotic liver diseases (MASLD). We used a combination of data-dependent acquisition (DDA) and data-independent acquisition (DIA or SWATH-MS) on SCIEX TripleTOF 6600, Metabolites and lipids were extracted using modified chloroform-methanol biphasic extraction methods for both biological matrices. Organic and aqueous fractions were separated using RRHD Eclipse Plus C18 and SeQuant ZIC-cHILIC columns prior to DDA and DIA acquisitions, respectively. All mzML files are deposited with ZENODO. *** DDA files are deposited in this dataset repository *** Child directories for SWATH data files Samples Polarity Zenodo DOI Organic fraction - Reverse Phase Positive & Negative 10.5281/zenodo.14096635 Aqueous fraction - HILIC Positive 10.5281/zenodo.14096753 Aqueous fraction - HILIC Negative 10.5281/zenodo.14136832