A comparative gene expression data set for functional Sfp1 knockdown mutants and wild type strains for five yeast Species in the LOG growth phase

This data set contains microarray data for wild type and Sfp1 mutant strains in each of several yeast species in the LOG growth phase. This was done at several time points (mainly the T=30, 60 or 90 min post glucose repletion) for S. cerevisiae, C. glabrata, N. castellii, K. lactis and S. pombe. For S. cerevisiae samples were taken for both WT and Sfp1 mutant conditions for both the BY and RM strains of this species. In S. cerevisiae, K. lactis and S. pombe there is one Sfp1 paralog and data were taken for the WT versions of these species and for a mutant in which Sfp1 was not functioning. This allows a comparison of the WT and mutant state in each species over several time points in the LOG growth phase. For S. cerevisiae both WT and Sfp1 mutant conditions were studied for both the BY and RM strains, as noted in the description of the samples and in the columns labels of the accompanying data matrix. For C. glabrata and N. Castellii there are two Sfp1 paralog genes, and data was taken for the individual mutant condition of each Sfp1 paralog in each species. These mutant data are noted with _9955 and _682 for the C. glabrata samples, and _719 and _511for the N. castellii samples. These designations indicate mutant conditions for either the CAGL0M09955g or CAGL0D00682g Sfp1 paralogs in C. glabrata, or likewise Scas719.24 or Scas551.1 in N. castellii. In the paper text N. castellii p719, C. glabrata p9995 are refered to as SFP1 and N. castellii p551 and C. glabrata paralog p0682 as SFP1PL. The over all design was to built a data set for the comparison of WT and Sfp1 mutant gene expression for several time points in the LOG growth phase for each of these yeast species to learn about 1) the importance of Sfp1 in the regulation of gene expression across these species in an evolutionary context and 2) to under stand the phenotypic implications for Sfp1 in evolution and the role of gene duplication in that evolution, notably with respect to the paralogs of Sfp1 in C. glabrata and N. castellii.