Notch1 binds Jag1

Cultured mouse endothelial cells expressing Notch1 are activated by immobilized mouse Jag1, as demonstrated by expression of Notch target genes Hes1 and Hey1. The activation of Notch1 by Jag1 is confirmed by a co-culture assay in which Jag1-expressing cells are mixed with Notch1-expressing cells transfected with the Notch reporter construct.

培养的携带Notch1蛋白表达的鼠类内皮细胞，可通过固定化的鼠类Jag1蛋白得以激活，该激活过程通过Notch靶基因Hes1和Hey1的表达得以证实。Jag1蛋白对Notch1的激活作用，通过将表达Jag1蛋白的细胞与转染Notch报告构建体的表达Notch1蛋白的细胞共培养的实验得到了验证。