Immunolabeling of TGF-β-expressing cells.

Data on TGF-β1 is presented in 3 different locations, layer II and V of the cerebral cortex and the caudate putamen (CP). Because TGF-β2 is not expressed in the caudate putamen, TGF-β2-expressing cells were only counted in the cerebral cortex. Similarly, data on TGF-β3 are provided only for layer II of the cerebral cortex. In the upper row, the total number of TGF-β-expressing cells counted in a 400×200 µm rectangular-shaped area immediately outside the lesion is shown. The number of double-labeled cells was also counted in the same field, and the calculated ratios were averaged. One-way ANOVA did not indicate significant differences between brain regions for any of the markers.